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retroviral plhcx plasmid  (Addgene inc)


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    Structured Review

    Addgene inc retroviral plhcx plasmid
    Retroviral Plhcx Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plhcx+flag+mpkm2/pLHCX-FLAG-mPKM2+(Plasmid+%2344239)/pm41165759-327-7-10
    Average 93 stars, based on 7 article reviews
    retroviral plhcx plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Phenotypic selection with an intrabody library reveals an anti-apoptotic function of PKM2 requiring Mitofusin-1
    Article Snippet: Primary and immortalized WT and PKM2-deficient MEFs (PKM2 Δ/Δ ) were maintained in MEMα medium supplemented with 10% FBS, penicillin and streptomycin (Gibco-Invitrogen), and 0.1 mM of 2-mercaptoethanol [ ]. .. 293T cells were maintained in DMEM containing 10% (vol/vol) FBS and antibiotics. pLHCX-Flag-mPKM2 (Plasmid #42512) was obtained from Addgene. ..

    Article Title: Regeneration of glomerular metabolism and function by podocyte pyruvate kinase M2 in diabetic nephropathy
    Article Snippet: .. Mouse full-length PKM2 cDNA including a BamH1 cutting site is amplified from pLHCX-FLAG-mPKM2 plasmid by PCR. pLHCX-FLAG-mPKM2 was a gift from Lewis Cantley and Matthew Vander Heiden (Addgene plasmid 44239; http://n2t.net/addgene:44239 ; RRID: Addgene_44239). .. PCR products were extracted by QIAamp DNA Micro Kit (Qiagen, 56304).

    Article Title: Covalent Inhibition of Pyruvate Kinase M2 Reprograms Metabolic and Inflammatory Pathways in Hepatic Macrophages against Non-alcoholic Fatty Liver Disease
    Article Snippet: .. The full length cDNAs encoding PKM1 and PKM2 were cloned from pLHCX-Flag-mPKM1 (Plasmid #42511, Addgene) and pLHCX-Flag-mPKM2 (Plasmid #42512, Addgene) by PCR technique using forward primer: 5'- GTACGAATTCATGCCGAAGCCACACAGT-3'; reverse primer: 5'- GTACCTCGAGTCAAGGTACAGGCACTACAC-3'. ..

    Article Title: CD8+ T cell metabolic flexibility elicited by CD28-ARS2 axis-driven alternative splicing of PKM supports antitumor immunity
    Article Snippet: Transfection was performed using FuGENE 6 (Promega) and Opti-MEM (Gibco) according to the manufacturer’s recommendations. .. The helper plasmid pCL-Eco was a gift from Inder Verma (Addgene plasmid # 12371; http://n2t.net/addgene:12371 ; RRID:Addgene_12371). pLHCX-FLAG-mPKM2 was a gift from Lewis Cantley & Matthew Vander Heiden (Addgene plasmid # 44239; http://n2t.net/addgene:44239 ; RRID: Addgene_44239). .. The murine TCR OTI-2A.pMIG II plasmid was a gift from Dario Vignali (Addgene plasmid # 52111; http://n2t.net/addgene:52111 ; RRID: Addgene_52111).

    Amplification:

    Article Title: Regeneration of glomerular metabolism and function by podocyte pyruvate kinase M2 in diabetic nephropathy
    Article Snippet: .. Mouse full-length PKM2 cDNA including a BamH1 cutting site is amplified from pLHCX-FLAG-mPKM2 plasmid by PCR. pLHCX-FLAG-mPKM2 was a gift from Lewis Cantley and Matthew Vander Heiden (Addgene plasmid 44239; http://n2t.net/addgene:44239 ; RRID: Addgene_44239). .. PCR products were extracted by QIAamp DNA Micro Kit (Qiagen, 56304).

    Polymerase Chain Reaction:

    Article Title: Regeneration of glomerular metabolism and function by podocyte pyruvate kinase M2 in diabetic nephropathy
    Article Snippet: .. Mouse full-length PKM2 cDNA including a BamH1 cutting site is amplified from pLHCX-FLAG-mPKM2 plasmid by PCR. pLHCX-FLAG-mPKM2 was a gift from Lewis Cantley and Matthew Vander Heiden (Addgene plasmid 44239; http://n2t.net/addgene:44239 ; RRID: Addgene_44239). .. PCR products were extracted by QIAamp DNA Micro Kit (Qiagen, 56304).

    Article Title: Covalent Inhibition of Pyruvate Kinase M2 Reprograms Metabolic and Inflammatory Pathways in Hepatic Macrophages against Non-alcoholic Fatty Liver Disease
    Article Snippet: .. The full length cDNAs encoding PKM1 and PKM2 were cloned from pLHCX-Flag-mPKM1 (Plasmid #42511, Addgene) and pLHCX-Flag-mPKM2 (Plasmid #42512, Addgene) by PCR technique using forward primer: 5'- GTACGAATTCATGCCGAAGCCACACAGT-3'; reverse primer: 5'- GTACCTCGAGTCAAGGTACAGGCACTACAC-3'. ..

    Clone Assay:

    Article Title: Covalent Inhibition of Pyruvate Kinase M2 Reprograms Metabolic and Inflammatory Pathways in Hepatic Macrophages against Non-alcoholic Fatty Liver Disease
    Article Snippet: .. The full length cDNAs encoding PKM1 and PKM2 were cloned from pLHCX-Flag-mPKM1 (Plasmid #42511, Addgene) and pLHCX-Flag-mPKM2 (Plasmid #42512, Addgene) by PCR technique using forward primer: 5'- GTACGAATTCATGCCGAAGCCACACAGT-3'; reverse primer: 5'- GTACCTCGAGTCAAGGTACAGGCACTACAC-3'. ..

    Construct:

    Article Title: Oncogenic kinase-induced PKM2 tyrosine 105 phosphorylation converts non-oncogenic PKM2 to a tumor promoter and induces cancer stem-like cells
    Article Snippet: .. The constructs expressing kinases AXL (23945), EPHA2 (23926), FAK (23902), MET (23889) and TYRO3 (23916) in the pDONR223 vector were purchased from Addgene, and these genes were transferred into pLentiN (37444) vector by Gateway cloning. pLHCX vector, pLHCX-Flag-mPKM2, and pLHCX-Flag-mPKM2-Y105F plasmids were from Dr. Jing Chen’s laboratory ( 17 ). .. The mPKM2-Y105D mutation was induced using a Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers were mPKM2-Y105D FP, 5′-GACCCCATCCTCGACCGGCCCGTTG-3′, and mPKM2-Y105D RP, 5′-CAACGGGCCGGTCGAGGATGGGGTC-3′.

    Expressing:

    Article Title: Oncogenic kinase-induced PKM2 tyrosine 105 phosphorylation converts non-oncogenic PKM2 to a tumor promoter and induces cancer stem-like cells
    Article Snippet: .. The constructs expressing kinases AXL (23945), EPHA2 (23926), FAK (23902), MET (23889) and TYRO3 (23916) in the pDONR223 vector were purchased from Addgene, and these genes were transferred into pLentiN (37444) vector by Gateway cloning. pLHCX vector, pLHCX-Flag-mPKM2, and pLHCX-Flag-mPKM2-Y105F plasmids were from Dr. Jing Chen’s laboratory ( 17 ). .. The mPKM2-Y105D mutation was induced using a Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers were mPKM2-Y105D FP, 5′-GACCCCATCCTCGACCGGCCCGTTG-3′, and mPKM2-Y105D RP, 5′-CAACGGGCCGGTCGAGGATGGGGTC-3′.

    Cloning:

    Article Title: Oncogenic kinase-induced PKM2 tyrosine 105 phosphorylation converts non-oncogenic PKM2 to a tumor promoter and induces cancer stem-like cells
    Article Snippet: .. The constructs expressing kinases AXL (23945), EPHA2 (23926), FAK (23902), MET (23889) and TYRO3 (23916) in the pDONR223 vector were purchased from Addgene, and these genes were transferred into pLentiN (37444) vector by Gateway cloning. pLHCX vector, pLHCX-Flag-mPKM2, and pLHCX-Flag-mPKM2-Y105F plasmids were from Dr. Jing Chen’s laboratory ( 17 ). .. The mPKM2-Y105D mutation was induced using a Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) and the primers were mPKM2-Y105D FP, 5′-GACCCCATCCTCGACCGGCCCGTTG-3′, and mPKM2-Y105D RP, 5′-CAACGGGCCGGTCGAGGATGGGGTC-3′.



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